Biotech Company to Patent Fuel-Secreting Bacterium By MATTHEW L. WALD Published: September 13, 2010 Recommend Twitter Linkedin Sign In to E-Mail Print Reprints Share A biotech company plans to announce Tuesday that it has won a patent on a genetically altered bacterium that converts sunlight and carbon dioxide into ingredients of diesel fuel, a step that could provide a new pathway for making ethanol or a diesel replacement that skips several cumbersome and expensive steps in existing methods. Joule Unlimited The bacterium’s product, which it secretes like sweat, is a class of hydrocarbon molecules called alkanes that are chemically indistinguishable from the ones made in oil refineries. The organism can grow in bodies of water unfit for drinking or on land that is useless for farming, according to the company, Joule Unlimited of Cambridge, Mass. “We make very clean, sulfur-free hydrocarbons that drop directly into the existing infrastructure for the production of diesel fuel,” said William J. Sims, the chief executive of Joule. The object, he said, was not to be an alternative for fossil fuels, but “to become a viable replacement.” Joule said it was the first company to patent an organism that secretes hydrocarbon fuel made continuously, directly from sunlight. Other companies, including Amyris Biotechnologies of Emeryville, Calif., and LS9 of San Carlos, Calif., are working on organisms that will make fuel if fed sugar from corn or cellulosic sources, but Joule’s bacterium does not require any sugar. Another company, Aurora Algae of Alameda, Calif., said Monday that it had developed an algae-based platform for production of fuel, pharmaceuticals and other valuable chemicals. Development of a photosynthetic organism to make hydrocarbons is “an important step,” said Eric J. Toone, the deputy director for technology at the Advanced Research Projects Agency-Energy, a new agency within the Energy Department that makes grants for high-risk, high-reward projects. But Mr. Toone and others cautioned that there were other steps to be mastered before such a technology could be commercialized. The organism is a cyanobacterium, also known as blue-green algae, although it is technically not an algae. It produces the fuel using photosynthesis, the process that plants use to make sugars and other materials from water, carbon dioxide and sunlight. Alternative energy experts agree that photosynthesis is a promising avenue for biofuel research. The challenge is turning the resulting product into a fuel. Many companies are trying to develop an algae to do that job. But it requires energy to separate the algae from the water and then process the oil they make internally into a usable fuel. An organism that secretes the desired product directly avoids both problems. In a test in Leander, Tex., Joule’s bacteria strain produced ethanol. Different variants can also make polymers and other high-value chemicals that are ordinarily derived from petroleum, according to Joule. The system can run on the carbon dioxide in ordinary air but will do better using the exhaust from a power plant, once pollutants like sulfur and nitrogen oxides have been removed, according to the company. Joule said it would begin construction next year on a commercial plant, which it hopes will begin operations in 2012. The company predicts a yield of 15,000 gallons of diesel components per acre — far more fuel than an acre of corn grown for ethanol can produce. Mr. Sims says the pilot project covers a little less than five acres. Because the process is modular, he said, a full-scale factory would simply mean making multiple copies of a smaller setup. And with a small amount of refining, he said, the hydrocarbons can be converted to an ingredient of jet fuel. An independent expert, Matthew C. Posewitz, a professor at the Colorado School of Mines, said that making an organism that secreted hydrocarbons was “definitely one of the most active areas in the whole game right now.” He said that Joule did not yet have a proved process, but that it had strong research and development capabilities. “They have some extreme horsepower within that company,” he said.

Cambridge startup has tech to grow nutrients without farmland Boston Business Journal by Kyle Alspach Date: Wednesday, July 13, 2011, 1:45pm EDT - Last Modified: Wednesday, July 13, 2011, 2:38pm EDT One of the latest companies to come out of Flagship VentureLabs, Essentient Inc. of Cambridge, Mass., is working on an approach to producing nutrients for human consumption that doesn’t require arable land, Essentient CEO David Berry said in an interview. “This would change the way we think about nutrients, how people source nutrients at the end of the day,” said Berry, a partner at Cambridge-based Flagship Ventures . “We are not trying to change people’s way of living or anything along those lines. We are trying to offer a way to get nutrients independent from arable land.” Berry said the company isn’t ready to give further specifics about the approach, which has been developed since the founding of the company in early 2010 at VentureLabs. Berry previously served as CEO of Joule Unlimited Inc. and Theracrine Inc., two companies that also began in VentureLabs, the startup accelerator of Flagship Ventures. Berry compared the approach to starting Essentient to the founding of Joule, a company that says it has developed a process for producing renewable diesel and ethanol using sunlight, waste carbon dioxide, water and specialty microorganisms. Joule said in May that it had reached an agreement to have access to 1,200 acres in New Mexico for fuel production. The idea for Joule came simply out of a desire to produce renewable fuels without the need for biomass, and the technology was invented from there, Berry said. Likewise, Essentient began with the aim of finding a way to help solve the problem of global malnourishment while avoiding the problems associated with modern agriculture, he said. “Agriculture as it exists today uses 70 percent of world’s fresh water, and it still doesn’t provide baseline nutrition for the entire world,” Berry said. “We wondered if we could do something better.” Essentient currently has a staff of 15 and is located at 840 Memorial Dr. in Cambridge, Berry said. The company expects to hit key milestones in the third and fourth quarters, and will likely be ready to offer more details by the first quarter of 2012, he said. Essentient has received funding from Flagship but the specifics aren’t being disclosed. Flagship CEO Noubar Afeyan is serving as chairman of Essentient, and Flagship principal, Geoffrey von Maltzahn, is serving as vice president of corporate development. Clarification: An earlier online version of this story mischaracterized Essentient's product. The company is producing "nutrients," a spokeswoman said – not "food."